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ATAC QC component #868

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ATAC QC component #868

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VladimirShitov
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@VladimirShitov VladimirShitov commented Aug 27, 2024

Changelog

Add component for calculating QC metrics for ATAC data.

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Contributes to #398

Checklist before requesting a review

  • I have performed a self-review of my code

  • Conforms to the Contributor's guide

  • Check the correct box. Does this PR contain:

    • Breaking changes
    • New functionality
    • Major changes
    • Minor changes
    • Documentation
    • Bug fixes
  • Proposed changes are described in the CHANGELOG.md

  • CI tests succeed!

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@DriesSchaumont DriesSchaumont left a comment

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Small comments, but looking good overall! 👍

src/qc/calculate_atac_qc_metrics/config.vsh.yaml Outdated Show resolved Hide resolved
src/qc/calculate_atac_qc_metrics/config.vsh.yaml Outdated Show resolved Hide resolved
mdata = mu.read(par["input"])

logger.debug("Making var names unique")
mdata.var_names_make_unique()
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I think I want to avoid calling this here

atac.obs.drop(col, axis=1, inplace=True)

logger.info("Calculating QC metrics")
sc.pp.calculate_qc_metrics(atac, percent_top=None, log1p=False, inplace=True, layer=par["layer"])
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@DriesSchaumont DriesSchaumont Oct 4, 2024

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I want to avoid that things get overwritten by scanpy, could you pass a copy of the data?

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Did it manually

@VladimirShitov
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Tests are now failing due to incorrect scanpy version specification:
3.545 ERROR: No matching distribution found for scanpy~=1.10.4

Shouldn't be a problem on my side, I just use scanpy.yaml for installation now. Worth fixing elsewhere?

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2 participants